Presence of phosphodiester backbone, but not nucleobases, in the guide's 3' terminal region is necessary for RISC loading and target cleavage in vitro and in vivo. (academic.oup.com)

Authors: O'Reilly D … Khvorova A Journal: Nucleic Acids Research Published: 2026-01-05 DOI: https://doi.org/10.1093/nar/gkaf1438